General Information

  • ID:  hor002801
  • Uniprot ID:  P12285
  • Protein name:  R15 gamma peptide
  • Gene name:  NA
  • Organism:  Aplysia californica (California sea hare)
  • Family:  NA
  • Source:  Animal
  • Expression:  Expressed within the abdominal ganglion in neurons R15, RB(HE), the two L9(G) gill motoneurons, and L40 interneuron, all are parts of autonomic control circuit that contributes to implementing a central command to coordinate autonomic activity with escape
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Aplysia (genus), Aplysiidae (family), Aplysioidea (superfamily), Aplysiida (order), Tectipleura, Euthyneura, Heterobranchia (subclass), Gastropoda (class), Mollusca (phylum), Lophotrochozoa, Spiralia, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  NA
  • GO BP:  GO:0007218 neuropeptide signaling pathway
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  QDVRQQLRMFDDLVQLRKLIETPSVYPSEEDEARLYD
  • Length:  37(120-156)
  • Propeptide:  MDSAGLHINFRLSHVLTVVTCILYILPPTTTAYSLPAPGKAAFQHQLSKRDVSDGSAERRPYTRMGSGGLKLHCQVHPANCPGGLMVTKKSDLLGALLSRNSPSSYGLPSRDMSTAYKRQDVRQQLRMFDDLVQLRKLIETPSVYPSEEDEARLYD
  • Signal peptide:  MDSAGLHINFRLSHVLTVVTCILYIL
  • Modification:  T1 Pyrrolidone carboxylic acid
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  The alpha-1 peptide acts as an osmoregulatory peptide, increasing blood volume, and also modulates the activity of a set of cardiac motor neurons that control heart rate.
  • Mechanism:  Isoform R15-1 produces peptide R15 alpha-2 which is a 24 aa long spliced form of peptide R15 alpha-1 which is produced by isoform R15-2.
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-P12285-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor002801_AF2.pdbhor002801_ESM.pdb

Physical Information

Mass: 513933 Formula: C196H312N54O65S
Absent amino acids: CGHNW Common amino acids: DL
pI: 4.13 Basic residues: 5
Polar residues: 5 Hydrophobic residues: 11
Hydrophobicity: -88.92 Boman Index: -11910
Half-Life: 0.8 hour Half-Life Yeast: 10 min
Half-Life E.Coli: >10 hour Aliphatic Index 89.46
Instability Index: 8600.27 Extinction Coefficient cystines: 2980
Absorbance 280nm: 82.78

Literature

  • PubMed ID:  17228083
  • Title:  Autonomic Control Network Active in Aplysia During Locomotion Includes Neurons That Express Splice Variants of R15-neuropeptides